Figure 1. Bai1−/− mice have more uncleared corpses after apoptosis induction in three different tissues.
(A) Engulfment of apoptotic thymocytes by BMDM from control and Bai1−/− mice were determined by flow cytometry. Cytochalasin D (CytoD) inhibits engulfment but not binding.
(B) Cleaved caspase-3 staining of thymus from Bai1+/+ and Bai1−/− mice after dexamethasone treatment (left) and its quantitation (right). (n=4 for Bai1+/+ and n=3 for Bai1−/−).
(C) Cleaved caspase-3 staining of testes from Bai1+/+ and Bai1−/− mice after sham treatment or testicular torsion (left) and its quantitation (right). (n=4; 4M for Bai1+/+, n=4; 4M for Bai1−/−).
(D) qPCR of Bai1, Timd4, and Stab2 in colon of Bai1+/+ (n=3) and Bai1−/− (n=3) mice (normalized to Gapdh). #, not detected.
(E) Schematic of the protocol for DSS-induced colitis and analysis.
(F) Apoptotic cells (TUNEL staining, red) in the proximal colonic region of Bai1+/+ (n=6; 6M) and Bai1−/− (n=5; 5M) mice at day 5 after DSS (5 %) treatment. Hoechst 33342 was used to normalize the total nuclei among different sections. Apoptotic index of Bai1+/+ was set to 100 %.
(G) Cleaved caspase-3 staining of proximal colon from Bai1+/+ and Bai1−/− mice after DSS (5 %) treatment (left) and quantitated (right). (n=8; 8M for Bai1+/+, n=7; 7M for Bai1−/−).
Data were from at least 2–3 independent experiments. Error bars indicate s.e.m. *P<0.05. M, male. Scale bars=50µm. See also Figures S1.