(Q33411668)

26 July 2017

title

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data (English)

1 reference

26 July 2017

1 reference

J M Ruijter

1 reference

26 July 2017

C Ramakers

1 reference

26 July 2017

W M H Hoogaars

1 reference

26 July 2017

Y Karlen

1 reference

26 July 2017

O Bakker

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26 July 2017

M J B van den Hoff

1 reference

26 July 2017

A F M Moorman

1 reference

26 July 2017

language of work or name

English

0 references

publication date

22 February 2009

1 reference

26 July 2017

published in

Nucleic Acids Research

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26 July 2017

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26 July 2017

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26 July 2017

page(s)

e45

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A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR.

26 July 2017

cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Revisiting the sigmoidal curve fitting applied to quantitative real-time PCR data

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Statistical significance of quantitative PCR

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Quantification of mRNA using real-time RT-PCR

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Quantitative real-time RT-PCR data analysis: current concepts and the novel "gene expression's CT difference" formula

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Comprehensive algorithm for quantitative real-time polymerase chain reaction

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Standardized determination of real-time PCR efficiency from a single reaction set-up

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Kinetic Outlier Detection (KOD) in real-time PCR.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

A new mathematical model for relative quantification in real-time RT-PCR

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Quantitative RT-PCR: pitfalls and potential

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Efficiency of DNA replication in the polymerase chain reaction

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Theoretical uncertainty of measurements using quantitative polymerase chain reaction.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Error propagation in relative real-time reverse transcription polymerase chain reaction quantification models: the balance between accuracy and precision.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Mathematical model of real-time PCR kinetics

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Validation of an algorithm for automatic quantification of nucleic acid copy numbers by real-time polymerase chain reaction

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

Validation of a quantitative method for real time PCR kinetics

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665230

High-resolution semi-quantitative real-time PCR without the use of a standard curve

31 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/19237396

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1093/NAR/GKP045

1 reference

26 July 2017

1 reference

26 July 2017

1 reference