Sodium/hydrogen exchanger 8: Difference between revisions

{{Infobox_gene}}

'''Sodium/hydrogen exchanger 8''' is a [[protein]] that in humans is encoded by the ''SLC9A8'' [[gene]].<ref name="pmid12409279">{{cite journal |author1=Goyal S |author2=Vanden Heuvel G |author3=Aronson PS | title = Renal expression of novel Na+/H+ exchanger isoform NHE8 | journal = Am J Physiol Renal Physiol | volume = 284 | issue = 3 | pages = F467–73 |date=Jan 2003 | pmid = 12409279 | doi = 10.1152/ajprenal.00352.2002 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: SLC9A8 solute carrier family 9 (sodium/hydrogen exchanger), member 8| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=23315}}</ref>

==Model organisms==

{| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: right;" |

|+ ''Slc9a8'' knockout mouse phenotype

|-

! Characteristic!! Phenotype

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| [[Homozygote]] viability || bgcolor="#488ED3"|Normal

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| Fertility || bgcolor="#488ED3"|Normal

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| Body weight || bgcolor="#488ED3"|Normal

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| [[Open Field (animal test)|Anxiety]] || bgcolor="#488ED3"|Normal

|-

| Neurological assessment || bgcolor="#488ED3"|Normal

|-

| Grip strength || bgcolor="#488ED3"|Normal

|-

| [[Hot plate test|Hot plate]] || bgcolor="#488ED3"|Normal

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| [[Dysmorphology]] || bgcolor="#488ED3"|Normal

|-

| [[Indirect calorimetry]] || bgcolor="#488ED3"|Normal

|-

| [[Glucose tolerance test]] || bgcolor="#488ED3"|Normal

|-

| [[Auditory brainstem response]] || bgcolor="#488ED3"|Normal

|-

| [[Dual-energy X-ray absorptiometry|DEXA]] || bgcolor="#488ED3"|Normal

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| [[Radiography]] || bgcolor="#488ED3"|Normal

|-

| Body temperature || bgcolor="#488ED3"|Normal

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| Eye morphology || bgcolor="#C40000"|Abnormal<ref name="Eye morphology">{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBFA/eye-morphology/ |title=Eye morphology data for Slc9a8 |publisher=Wellcome Trust Sanger Institute}}</ref>

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| [[Clinical chemistry]] || bgcolor="#488ED3"|Normal

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| [[Haematology]] || bgcolor="#488ED3"|Normal

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| [[Peripheral blood lymphocyte]]s || bgcolor="#488ED3"|Normal

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| [[Micronucleus test]] || bgcolor="#488ED3"|Normal

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| Heart weight || bgcolor="#488ED3"|Normal

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| Brain histopathology || bgcolor="#488ED3"|Normal

|-

| colspan=2; style="text-align: center;" | All tests and analysis from<ref name="mgp_reference">{{cite journal| doi = 10.1111/j.1755-3768.2010.4142.x| title = The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice| year = 2010| author = Gerdin AK| journal = Acta Ophthalmologica| volume = 88| issue = S248 | s2cid = 85911512}}</ref><ref>[http://www.sanger.ac.uk/mouseportal/ Mouse Resources Portal], Wellcome Trust Sanger Institute.</ref>

|}

[[Model organism]]s have been used in the study of SLC9A8 function. A conditional [[knockout mouse]] line, called ''Slc9a8^{tm1a(KOMP)Wtsi}''<ref name="allele_ref">{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Slc9a8 |title=International Knockout Mouse Consortium}}</ref><ref name="mgi_allele_ref">{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4363224 |title=Mouse Genome Informatics}}</ref> was generated as part of the [[International Knockout Mouse Consortium]] program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the [[Wellcome Trust Sanger Institute]].<ref name="pmid21677750">{{Cite journal

| last1 = Skarnes |first1 =W. C.

| doi = 10.1038/nature10163

| last2 = Rosen | first2 = B.

| last3 = West | first3 = A. P.

| last4 = Koutsourakis | first4 = M.

| last5 = Bushell | first5 = W.

| last6 = Iyer | first6 = V.

| last7 = Mujica | first7 = A. O.

| last8 = Thomas | first8 = M.

| last9 = Harrow | first9 = J.

| last10 = Cox | first10 = T.

| last11 = Jackson | first11 = D.

| last12 = Severin | first12 = J.

| last13 = Biggs | first13 = P.

| last14 = Fu | first14 = J.

| last15 = Nefedov | first15 = M.

| last16 = De Jong | first16 = P. J.

| last17 = Stewart | first17 = A. F.

| last18 = Bradley | first18 = A.

| title = A conditional knockout resource for the genome-wide study of mouse gene function

| journal = Nature

| volume = 474

| issue = 7351

| pages = 337–342

| year = 2011

| pmid = 21677750

| pmc =3572410

}}</ref><ref name="mouse_library">{{cite journal |author=Dolgin E |title=Mouse library set to be knockout |journal=Nature |volume=474 |issue=7351 |pages=262–3 |date=June 2011 |pmid=21677718 |doi=10.1038/474262a |doi-access=free }}</ref><ref name="mouse_for_all_reasons">{{cite journal |author1=Collins FS |author2=Rossant J |author3=Wurst W |title=A mouse for all reasons |journal=Cell |volume=128 |issue=1 |pages=9–13 |date=January 2007 |pmid=17218247 |doi=10.1016/j.cell.2006.12.018 |s2cid=18872015 |doi-access=free }}</ref>

Male and female animals underwent a standardized [[phenotypic screen]] to determine the effects of deletion.<ref name="mgp_reference" /><ref name="pmid21722353">{{cite journal|author1=van der Weyden L |author2=White JK |author3=Adams DJ |author4=Logan DW | title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | pmc=3218837}}</ref> Twenty one tests were carried out on [[mutant]] mice and one significant abnormality was observed: [[homozygous]] mutant animals had abnormal [[retina]]l morphology and pigmentation.<ref name="mgp_reference" />