Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts (Q58575187)

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29 April 2020

title

Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts (English)

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29 April 2020

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29 April 2020

Laiyou Wang

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29 April 2020

Aihua Deng

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29 April 2020

Yun Zhang

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29 April 2020

Shuwen Liu

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29 April 2020

Yong Liang

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29 April 2020

Hua Bai

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29 April 2020

Di Cui

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29 April 2020

Qidi Qiu

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29 April 2020

Xiuling Shang

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29 April 2020

Zhao Yang

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29 April 2020

Xiuping He

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29 April 2020

language of work or name

English

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publication date

10 October 2018

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Biotechnology for Biofuels

29 April 2020

published in

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volume

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issue

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page(s)

277

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Creative Commons Attribution 4.0 International

29 April 2020

start time

10 October 2018

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April 2022 Public Data File from Crossref

copyrighted

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cites work

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30 November 2018

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A new set of rDNA-NTS-based multiple integrative cassettes for the development of antibiotic-marker-free recombinant yeasts

30 November 2018

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Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system

30 November 2018

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Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

30 November 2018

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Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

30 November 2018

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Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

30 November 2018

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30 November 2018

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Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances

30 November 2018

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A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae.

30 November 2018

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De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae

30 November 2018

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Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus

30 November 2018

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Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites.

30 November 2018

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CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae.

30 November 2018

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Generation of hepatitis B virus PreS2-S antigen in Hansenula polymorpha

30 November 2018

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Genome editing. The new frontier of genome engineering with CRISPR-Cas9

30 November 2018

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Implementation of the CRISPR-Cas9 system in fission yeast

30 November 2018

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Scarless gene deletion in methylotrophic Hansenula polymorpha by using mazF as counter-selectable marker.

30 November 2018

1 reference

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Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae

30 November 2018

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Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation.

30 November 2018

1 reference

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DNA targeting specificity of RNA-guided Cas9 nucleases

30 November 2018

1 reference

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Genome-wide analysis of intraspecific transposon diversity in yeast

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

A mimicking-of-DNA-methylation-patterns pipeline for overcoming the restriction barrier of bacteria

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Novel genetic tools for Hansenula polymorpha

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Improved gene disruption method and Cre-loxP mutant system for multiple gene disruptions in Hansenula polymorpha.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

New selectable host-marker systems for multiple genetic manipulations based on TRP1, MET2 and ADE2 in the methylotrophic yeast Hansenula polymorpha

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Uricase production by a recombinant Hansenula polymorpha strain harboring Candida utilis uricase gene

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Expression and purification of human endostatin from Hansenula polymorpha A16.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Comparison of two alternative dominant selectable markers for wine yeast transformation

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Single-step co-integration of multiple expressible heterologous genes into the ribosomal DNA of the methylotrophic yeast Hansenula polymorpha

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

One-step, PCR-mediated, gene disruption in the yeast Hansenula polymorpha.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Yeasts have a four-fold variation in ribosomal DNA copy number

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement.

30 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6180501

High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression

30 November 2018

1 reference

12 December 2020

Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes

1 reference

12 December 2020

Highly-efficient electrotransformation of the yeast Hansenula polymorpha

1 reference

12 December 2020

Overview of N- and O-linked oligosaccharide structures found in various yeast species

1 reference

12 December 2020

Vectors for rapid selection of integrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1

1 reference

12 December 2020

A dominant selection system designed for copy-number-controlled gene integration in Hansenula polymorpha DL-1

1 reference

12 December 2020

Construction of integrative plasmids suitable for genetic modification of industrial strains of Saccharomyces cerevisiae

1 reference

12 December 2020

10.1186/S13068-018-1271-0

Identifiers

DOI

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Dimensions Publication ID

29 April 2020

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29 April 2020

PubMed publication ID

30337956

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