Genetically encoding an aliphatic diazirine for protein photocrosslinking (Q57437722)

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Rajendra Uprety

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Lloyd Davis

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Jason W. Chin

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Alexander Deiters

New photolabeling and crosslinking methods

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publication date

2011

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480-483

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Benzophenone photophores in biochemistry.

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Photocrosslinking and click chemistry enable the specific detection of proteins interacting with phospholipids at the membrane interface

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Photo-leucine incorporation reveals the target of a cyclodepsipeptide inhibitor of cotranslational translocation

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Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars.

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Photocrosslinking in ruthenium-labelled duplex oligonucleotides.

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A novel strategy for the identification of protein-DNA contacts by photocrosslinking and mass spectrometry

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Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cells

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Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid

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Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane.

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Probing the environment along the protein import pathways in yeast mitochondria by site-specific photocrosslinking

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Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli

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Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli

21 January 2018

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In vivo photocrosslinking with unnatural amino Acid mutagenesis

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A genetically encoded diazirine photocrosslinker in Escherichia coli

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A generic protein purification method for protein complex characterization and proteome exploration

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An expanded eukaryotic genetic code

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Coupled chaperone action in folding and assembly of hexadecameric Rubisco

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Two cooperating helices constitute the lipid-binding domain of the bacterial SRP receptor.

21 January 2018

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Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB.

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M domains couple the ClpB threading motor with the DnaK chaperone activity

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The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex.

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Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking

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Real-time observation of trigger factor function on translating ribosomes.

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Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB.

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Reaching for high-hanging fruit in drug discovery at protein-protein interfaces

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Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

21 January 2018

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Genetically encoding N(epsilon)-acetyllysine in recombinant proteins.

21 January 2018

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A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli.

21 January 2018

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Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

21 January 2018

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A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

21 January 2018

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A facile system for encoding unnatural amino acids in mammalian cells

21 January 2018

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Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification

21 January 2018

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Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry.

21 January 2018

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Genetically encoding N(epsilon)-methyl-L-lysine in recombinant histones.

21 January 2018

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Genetically encoded photocontrol of protein localization in mammalian cells

21 January 2018

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Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV

21 January 2018

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Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion.

21 January 2018

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Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair

21 January 2018

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