Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases (Q42375190)

28 October 2017

Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases (English)

1 reference

28 October 2017

Dingbo Zhang

1 reference

28 October 2017

Huawei Zhang

1 reference

28 October 2017

Tingdong Li

1 reference

28 October 2017

Kunling Chen

1 reference

28 October 2017

Jin-Long Qiu

1 reference

28 October 2017

Caixia Gao

1 reference

28 October 2017

11 October 2017

1 reference

28 October 2017

1 reference

28 October 2017

1 reference

28 October 2017

1 reference

28 October 2017

191

1 reference

Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery.

28 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Guide RNA engineering for versatile Cas9 functionality

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Rationally engineered Cas9 nucleases with improved specificity

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Small molecule-triggered Cas9 protein with improved genome-editing specificity

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

In vivo genome editing using Staphylococcus aureus Cas9

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

A split-Cas9 architecture for inducible genome editing and transcription modulation

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Precision genome engineering and agriculture: opportunities and regulatory challenges

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Development and applications of CRISPR-Cas9 for genome engineering

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Targeted genome modification of crop plants using a CRISPR-Cas system.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

DNA targeting specificity of RNA-guided Cas9 nucleases

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

RNA-guided human genome engineering via Cas9

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Multiplex genome engineering using CRISPR/Cas systems

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Mapping the genomic landscape of CRISPR-Cas9 cleavage.

28 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

16 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

16 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

Genome editing in rice and wheat using the CRISPR/Cas system

16 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants.

16 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5637269

10.1186/S13059-017-1325-9

16 August 2018

Identifiers

DOI

1 reference

Dimensions Publication ID

28 October 2017

1 reference

9 September 2019

https://scigraph.springernature.com/pub.10.1186/s13059-017-1325-9

1 reference

28 October 2017

PubMed publication ID

29020979

1 reference