Heterologous expression of the plant cysteine protease bromelain and its inhibitor in Pichia pastoris. (Q40446201)

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27 March 2020

title

Heterologous expression of the plant cysteine protease bromelain and its inhibitor in Pichia pastoris (English)

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Pichia pastoris

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Nora Luniak

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Sonja Burkart

series ordinal

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publication date

29 November 2016

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published in

Biotechnology Progress

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volume

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issue

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page(s)

54-65

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Bromelain protease F9 reduces the CD44 mediated adhesion of human peripheral blood lymphocytes to human umbilical vein endothelial cells

27 March 2020

cites work

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Stability, purification, and applications of bromelain: A review.

21 January 2018

1 reference

Isolation and characterization of two forms of an acidic bromelain stem proteinase

21 January 2018

1 reference

Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities

21 January 2018

1 reference

Ananain: a novel cysteine proteinase found in pineapple stem

21 January 2018

1 reference

Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases

21 January 2018

1 reference

Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.

21 January 2018

1 reference

Bromelain: biochemistry, pharmacology and medical use.

21 January 2018

1 reference

Bromelain activates murine macrophages and natural killer cells in vitro.

21 January 2018

1 reference

A novel yeast expression/secretion system for the recombinant plant thiol endoprotease propapain

21 January 2018

1 reference

Solution structure of bromelain inhibitor IV from pineapple stem: structural similarity with Bowman-Birk trypsin/chymotrypsin inhibitor from soybean

21 January 2018

1 reference

Characterization of genomic sequence coding for bromelain inhibitors in pineapple and expression of its recombinant isoform

21 January 2018

1 reference

CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design

21 January 2018

1 reference

Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena

21 January 2018

1 reference

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

21 January 2018

1 reference

Active site titration of cysteine proteases

21 January 2018

1 reference

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

21 January 2018

1 reference

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites

21 January 2018

1 reference

PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization

21 January 2018

1 reference

Improvements in protein secondary structure prediction by an enhanced neural network

21 January 2018

1 reference

Probability-based protein identification by searching sequence databases using mass spectrometry data

21 January 2018

1 reference

Structure-function relationship of bromelain isoinhibitors from pineapple stem

21 January 2018

1 reference

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

21 January 2018

1 reference

Heterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems

21 January 2018

1 reference

The expression in Escherichia coli of a synthetic gene coding for the precursor of papain is prevented by its own putative signal sequence

21 January 2018

1 reference

Recombinant pro-regions from papain and papaya proteinase IV-are selective high affinity inhibitors of the mature papaya enzymes.

21 January 2018

1 reference

Two distinct gene subfamilies within the family of cysteine protease genes

21 January 2018

1 reference

21 January 2018

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Signal-mediated retrieval of a membrane protein from the Golgi to the ER in yeast

21 January 2018

1 reference

Functions of Propeptide Parts in Cysteine Proteases

21 January 2018

1 reference

Susceptibility of the interchain peptide of a bromelain inhibitor precursor to the target proteases bromelain, chymotrypsin, and trypsin

21 January 2018

1 reference

Bromelain's activity and potential as an anti-cancer agent: Current evidence and perspectives

21 January 2018

1 reference

Bromelain as a Treatment for Osteoarthritis: a Review of Clinical Studies

21 January 2018

1 reference

Placebo-controlled randomized clinical trial on the immunomodulating activities of low- and high-dose bromelain after oral administration - new evidence on the antiinflammatory mode of action of bromelain.

21 January 2018

1 reference

Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. An update

21 January 2018

1 reference

Strains and molecular tools for recombinant protein production in Pichia pastoris

21 January 2018

1 reference

Heterologous gene expression in filamentous fungi

21 January 2018

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Production of recombinant proteins by filamentous fungi

21 January 2018

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Production and activation of recombinant papain-like cysteine proteases

21 January 2018

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Folding at the rhythm of the rare codon beat

21 January 2018

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Functional characterization of recombinant bromelain of Ananas comosus expressed in a prokaryotic system

21 January 2018

1 reference

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain.

21 January 2018

1 reference

Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology.

21 January 2018

1 reference

Specific molten globule conformation of stem bromelain at alkaline pH.

21 January 2018

1 reference

21 January 2018

Identifiers

DOI

10.1002/BTPR.2405

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27 March 2020

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