Transient kinetics, fluorescence, and FRET in studies of initiation of translation in bacteria (Q46968954)

27 December 2017

title

Transient kinetics, fluorescence, and FRET in studies of initiation of translation in bacteria (English)

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27 December 2017

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27 December 2017

Andrey L. Konevega

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27 December 2017

Attilio Fabbretti

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27 December 2017

Marina Rodnina

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27 December 2017

author name string

Frank Peske

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27 December 2017

Claudio O Gualerzi

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27 December 2017

language of work or name

English

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publication date

1 January 2007

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27 December 2017

published in

Methods in Enzymology

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27 December 2017

volume

430

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27 December 2017

page(s)

1-30

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27 December 2017

The cryo-EM structure of a translation initiation complex from Escherichia coli

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How initiation factors tune the rate of initiation of protein synthesis in bacteria

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Ribosome formation from subunits studied by stopped-flow and rayleigh light scattering

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X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix

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Post-transcriptional regulation of CspA expression in Escherichia coli

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The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli.

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Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase

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Chemical synthesis and in vivo hyperexpression of a modular gene coding for Escherichia coli translational initiation factor IF1.

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Mechanism of translational initiation in prokaryotes

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7 January 2021

Crystal structure of an initiation factor bound to the 30S ribosomal subunit

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7 January 2021

Translation initiation factor IF2 interacts with the 30 S ribosomal subunit via two separate binding sites

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7 January 2021

Interaction of Escherichia coli translation-initiation factor IF-1 with ribosomes.

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7 January 2021

Interaction of translation initiation factor IF1 with the E. coli ribosomal A site.

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7 January 2021

Interaction of translation initiation factor 3 with the 30S ribosomal subunit

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7 January 2021

Translational standby sites: how ribosomes may deal with the rapid folding kinetics of mRNA.

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7 January 2021

Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations.

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7 January 2021

The real-time path of translation factor IF3 onto and off the ribosome.

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7 January 2021

Solution structure of the ribosome-binding domain of E. coli translation initiation factor IF3. Homology with the U1A protein of the eukaryotic spliceosome.

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7 January 2021

Preferential translation of cold-shock mRNAs during cold adaptation

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7 January 2021

Kinetic determinants of high-fidelity tRNA discrimination on the ribosome

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7 January 2021

Kinetic mechanism of elongation factor Ts-catalyzed nucleotide exchange in elongation factor Tu.

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7 January 2021

Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes

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7 January 2021

Initial rate kinetic analysis of the mechanism of initiation complex formation and the role of initiation factor IF-3

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7 January 2021

Initiation factors in the early events of mRNA translation in bacteria

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7 January 2021

Initiation of mRNA translation in prokaryotes

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7 January 2021

A time-resolved investigation of ribosomal subunit association.

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7 January 2021

Translational Operator of mRNA on the Ribosome: How Repressor Proteins Exclude Ribosome Binding

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7 January 2021

Distance moved by transfer RNA during translocation from the A site to the P site on the ribosome

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7 January 2021

Novel roles for classical factors at the interface between translation termination and initiation

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7 January 2021

The importance of structural transitions of the switch II region for the functions of elongation factor Tu on the ribosome

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7 January 2021

Single-step purification of specific tRNAs by hydrophobic tagging.

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7 January 2021

Prokaryotic translation initiation factor IF3 is an elongated protein consisting of two crystallizable domains

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7 January 2021

Translation of mRNAs with degenerate initiation triplet AUU displays high initiation factor 2 dependence and is subject to initiation factor 3 repression

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7 January 2021

The NH2-terminal cleavage of Escherichia coli translational initiation factor IF3. A mechanism to control the intracellular level of the factor?

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7 January 2021

Initiation of protein synthesis in bacteria

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7 January 2021

Ribosomal localization of translation initiation factor IF2

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7 January 2021

Location of translational initiation factor IF3 on the small ribosomal subunit

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7 January 2021

The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor

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7 January 2021

Specific protection of 16 S rRNA by translational initiation factors

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7 January 2021

Conformational transition of initiation factor 2 from the GTP- to GDP-bound state visualized on the ribosome.

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7 January 2021

Hydrogen exchange studies on ribosomes.

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7 January 2021

Sequence of steps in ribosome recycling as defined by kinetic analysis.

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7 January 2021

Mapping the active sites of bacterial translation initiation factor IF3.

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7 January 2021

Translation initiation factor IF3: two domains, five functions, one mechanism?

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7 January 2021

Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.

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7 January 2021

Kinetic Analysis of Interaction of Eukaryotic Release Factor 3 with Guanine Nucleotides

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7 January 2021

Effect of Initiation Factor 3 Binding on the 30S Ribosomal Subunits of Escherichia coli

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7 January 2021

18 Qualitative and semiquantitative assay of Escherichia coli translational initiation factor IF-3

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7 January 2021

Mechanism of protein biosynthesis in prokaryotic cells. Effect of initiation factor IF1 on the initial rate of 30 S initiation complex formation

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7 January 2021

Mechanism of translational initiation in prokaryotes. IF3 is released from ribosomes during and not before 70 S initiation complex formation.

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7 January 2021

Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication

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7 January 2021

Specificity and Properties of the Destabilization, Induced by Initiation Factor IF-3, of Ternary Complexes of the 30-S Ribosomal Subunit, Aminoacyl-tRNA and Polynucleotides

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7 January 2021

Transient conformational states of aminoacyl-tRNA during ribosome binding catalyzed by elongation factor Tu.

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7 January 2021

Thiostrepton inhibits the turnover but not the GTPase of elongation factor G on the ribosome.

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7 January 2021

Purification of fMet-tRNA(fMet) by fast protein liquid chromatography.

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7 January 2021

GTPases mechanisms and functions of translation factors on the ribosome

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding

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7 January 2021

An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation

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7 January 2021

The structure of the translational initiation factor IF1 from E.coli contains an oligomer-binding motif

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7 January 2021

Unfolding of mRNA secondary structure by the bacterial translation initiation complex

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7 January 2021

Late events of translation initiation in bacteria: a kinetic analysis

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7 January 2021

Role and timing of GTP binding and hydrolysis during EF-G-dependent tRNA translocation on the ribosome

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7 January 2021

Effect of Escherichia coli initiation factors on the kinetics of N-AcPhe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study

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7 January 2021

53 Replacement of odd bases in tRNA by fluorescent dyes

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7 January 2021

Kinetics of the reversible association of ribosomal subunits: Stopped-flow studies of the rate law and of the effect of Mg2+

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7 January 2021

Structural basis for messenger RNA movement on the ribosome

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7 January 2021

The path of messenger RNA through the ribosome

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7 January 2021

Magnesium dependence and equilibrium of the Escherichia coli ribosomal subunit association

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7 January 2021

10.1016/S0076-6879(07)30001-3

Identifiers

DOI

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27 December 2017

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27 December 2017