Optimized RNA ISH, RNA FISH and protein-RNA double labeling (IF/FISH) in Drosophila ovaries (Q30586225)

18 October 2017

Optimized RNA ISH, RNA FISH and protein-RNA double labeling (IF/FISH) in Drosophila ovaries (English)

1 reference

18 October 2017

0 references

0 references

Sandra G Zimmerman

0 references

Nathaniel C Peters

1 reference

18 October 2017

Ariel E Altaras

series ordinal

1 reference

18 October 2017

Celeste A Berg

series ordinal

1 reference

18 October 2017

language of work or name

1 reference

10 October 2013

1 reference

18 October 2017

1 reference

18 October 2017

1 reference

18 October 2017

1 reference

18 October 2017

2158-2179

1 reference

describes a project that uses

18 October 2017

ImageJ

1 reference

11 June 2022

https://www.ebi.ac.uk/europepmc/webservices/rest/PMC4126239/fullTextXML

based on heuristic

inferred from PubMed Central ID database lookup

cites work

Protein equilibration through somatic ring canals in Drosophila.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Following the 'tracks': Tramtrack69 regulates epithelial tube expansion in the Drosophila ovary through Paxillin, Dynamin, and the homeobox protein Mirror

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis

20 June 2018

1 reference

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Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration.

20 June 2018

1 reference

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Steroid-induced microRNA let-7 acts as a spatio-temporal code for neuronal cell fate in the developing Drosophila brain

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

High-throughput RNA in situ hybridization in mouse retina

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Dual fluorescence detection of protein and RNA in Drosophila tissues.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Optimizing the specificity of nucleic acid hybridization

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Transcriptional interpretation of the EGF receptor signaling gradient

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Gene regulation by MAPK substrate competition.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

In situ detection and genotyping of individual mRNA molecules

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Lighting up mRNA localization in Drosophila oogenesis

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Determination of gene expression patterns using high-throughput RNA in situ hybridization to whole-mount Drosophila embryos

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

A combinatorial code for pattern formation in Drosophila oogenesis

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

In situ hybridization analysis of chick embryos in whole-mount and tissue sections.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

dGRASP-mediated noncanonical integrin secretion is required for Drosophila epithelial remodeling

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

High-resolution in situ hybridization to whole-mount zebrafish embryos

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Global analysis of patterns of gene expression during Drosophila embryogenesis

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

High-throughput functional screen of mouse gastrula cDNA libraries reveals new components of endoderm and mesoderm specification

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Multiplex detection of RNA expression in Drosophila embryos

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Large-scale expression screening by automated whole-mount in situ hybridization

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Systematic determination of patterns of gene expression during Drosophila embryogenesis

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Profiling patterned transcripts in Drosophila embryos

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Transcribed genes are localized according to chromosomal position within polarized Drosophila embryonic nuclei

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Single and double FISH protocols for Drosophila

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Comparison of in-situ hybridization, direct and indirect in-situ PCR as well as tyramide signal amplification for the detection of HPV.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Visualization of single RNA transcripts in situ

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Gene Copy Number Analysis by Fluorescencein SituHybridization and Comparative Genomic Hybridization

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Isoform-specific 3'-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Double-label in situ hybridization using biotin and digoxigenin-tagged RNA probes.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

The drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGFα-like protein

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

In situ hybridization to RNA.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Padlock Probes: Circularizing Oligonucleotides for Localized DNA Detection

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Molecular hybridization of radioactive DNA to the DNA of cytological preparations

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Formation and detection of RNA-DNA hybrid molecules in cytological preparations

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Drosophilia spectrin. I. Characterization of the purified protein

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Expression of an engrailed-related protein is induced in the anterior neural ectoderm of early Xenopus embryos.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Oskar organizes the germ plasm and directs localization of the posterior determinant nanos

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Role of neurogenic genes in establishment of follicle cell fate and oocyte polarity during oogenesis in Drosophila

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Appendix G: In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Localizing DNA and RNA within nuclei and chromosomes by fluorescence in situ hybridization

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction

20 June 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Simultaneous detection of RNA and protein by in situ hybridization and immunological staining.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Determination of gene expression patterns using in situ hybridization to Drosophila testes.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Homeless is required for RNA localization in Drosophila oogenesis and encodes a new member of the DE-H family of RNA-dependent ATPases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Rap-GEF signaling controls stem cell anchoring to their niche through regulating DE-cadherin-mediated cell adhesion in the Drosophila testis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Glycosphingolipids control the extracellular gradient of the Drosophila EGFR ligand Gurken.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Boss/Sev signaling from germline to soma restricts germline-stem-cell-niche formation in the anterior region of Drosophila male gonads

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

In Situ Hybridization to Somatic Chromosomes in Drosophila

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Multicolor whole-mount in situ hybridization.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Hybridization to tissues in suspension for whole-mount FISH in Drosophila.

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Manual dissection and fixation of Drosophila egg chambers for whole-mount FISH.

26 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Fragmentation and labeling of probe DNA for whole-mount FISH in Drosophila.

26 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Fluorescent in situ hybridization protocols in Drosophila embryos and tissues

26 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

Oskar anchoring restricts pole plasm formation to the posterior of the Drosophila oocyte

26 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126239

An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies

26 November 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

In Situ Hybridization to Drosophila Testes: Figure 1

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

High-resolution fluorescent in situ hybridization of Drosophila embryos and tissues

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

Synthesis and purification of digoxigenin-labeled RNA probes for in situ hybridization

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2013.136

31 March 2020

Identifiers

DOI

10.1038/NPROT.2013.136

1 reference

Dimensions Publication ID

18 October 2017

0 references

1 reference

18 October 2017

PubMed publication ID

24113787

1 reference