Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA. (Q39542739)

12 September 2017

Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA. (English)

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12 September 2017

J J Li

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12 September 2017

R Geyer

series ordinal

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12 September 2017

W Tan

series ordinal

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12 September 2017

language of work or name

English

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publication date

1 June 2000

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12 September 2017

Nucleic Acids Research

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12 September 2017

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12 September 2017

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12 September 2017

E52

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Multiplex detection of four pathogenic retroviruses using molecular beacons

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Real time detection of DNA.RNA hybridization in living cells

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Molecular beacons: probes that fluoresce upon hybridization

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Real time kinetics of restriction endonuclease cleavage monitored by fluorescence resonance energy transfer

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

A fluorometric assay for DNA cleavage reactions characterized with BamHI restriction endonuclease

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Nuclease footprint analyses of the interactions between RNase P ribozyme and a model mRNA substrate

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Drunken-cell footprints: nuclease treatment of ethanol-permeabilized bacteria reveals an initiation-like nucleoprotein complex in stationary phase replication origins

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Effects of prostaglandins and some anti-inflammatory drugs on the binding of 7,12-dimethylbenz[a]anthracene to the DNA of murine epidermal cells in culture

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Specificity of the S1 nuclease from Aspergillus oryzae

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Oxidative chemical nucleases.

25 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Fluorescence assays for DNA cleavage

25 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Kinetic studies of the hydrolysis of platinum-DNA complexes by nuclease S1.

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

New antiviral strategy using capsid-nuclease fusion proteins

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Continuous spectrophotometric assay for restriction endonucleases using synthetic oligodeoxynucleotides and based on the hyperchromic effect

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Molecular Beacons: A Novel Approach to Detect Protein - DNA Interactions This work was partially supported by a U.S. NSF Career Award (CHE-9733650) and by a U.S. Office of Naval Research Young Investigator Award (N00014-98-1-0621).

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Intracellular mRNA cleavage induced through activation of RNase P by nuclease-resistant external guide sequences.

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Probing the mechanism of staphylococcal nuclease with unnatural amino acids: kinetic and structural studies

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Sequence-specific scission of DNA by RNAs linked to a chemical nuclease

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Chemical conversion of a DNA-binding protein into a site-specific nuclease

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

A Fast and Accurate Enzyme-Linked Immunosorbent Assay for the Determination of the DNA Cleavage Activity of Restriction Endonucleases

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=102637

Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis

2 December 2018

1 reference

12 December 2020

A single nuclease active site of the Escherichia coli RecBCD enzyme catalyzes single-stranded DNA degradation in both directions

1 reference

12 December 2020

Characterization of endonucleolytic activity of HIV-1 integrase using a fluorogenic substrate

1 reference

12 December 2020

Kinetic parameters of superhelical DNA cleavage by endonuclease S1

1 reference

12 December 2020

Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonuclease

1 reference

12 December 2020

Molecular design of artificial hydrolytic nucleases and ribonucleases

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12 December 2020

Scission of DNA at a preselected sequence using a single-strand-specific chemical nuclease

1 reference

12 December 2020

Single-strand-specific nucleases

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12 December 2020

Identifiers

DOI

10.1093/NAR/28.11.E52

1 reference

12 September 2017

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12 September 2017

PubMed publication ID

10871351

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