Immunolabeling artifacts and the need for live-cell imaging (Q39404118)

11 September 2017

title

Immunolabeling artifacts and the need for live-cell imaging (English)

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11 September 2017

Ulrike Schnell

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11 September 2017

Freark Dijk

series ordinal

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11 September 2017

Klaas A Sjollema

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11 September 2017

Ben N G Giepmans

series ordinal

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11 September 2017

publication date

30 January 2012

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11 September 2017

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11 September 2017

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11 September 2017

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11 September 2017

page(s)

152-158

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The fluorescent toolbox for assessing protein location and function

11 September 2017

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Comparison of fixation protocols for adherent cultured cells applied to a GFP fusion protein of the epidermal growth factor receptor

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Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins

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Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins

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Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions

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Immunofluorescence imaging of the influenza virus M1 protein is dependent on the fixation method

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Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells.

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Comparison of intra-organellar chaperone capacity for dealing with stress-induced protein unfolding

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Membrane molecules mobile even after chemical fixation

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Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy

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12 December 2020

Pre-embedding immunolabeling for electron microscopy: an evaluation of permeabilization methods and markers

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12 December 2020

Overview of cell fixatives and cell membrane permeants

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12 December 2020

Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins

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12 December 2020

Routine formaldehyde fixation irreversibly reduces immunoreactivity of Bcl-2 in the nuclear compartment of breast cancer cells, but not in the cytoplasm

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12 December 2020

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12 December 2020

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12 December 2020

Differential extraction of proteins from paraformaldehyde-fixed cells: lessons from synaptophysin and other membrane proteins

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12 December 2020

Effects of acetone, methanol, or paraformaldehyde on cellular structure, visualized by reflection contrast microscopy and transmission and scanning electron microscopy.

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12 December 2020

Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells

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12 December 2020

A simple fixation procedure for immunofluorescent detection of different cytoskeletal components within the same cell

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12 December 2020

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12 December 2020

Identifiers

DOI

10.1038/NMETH.1855

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11 September 2017

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