Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications (Q39025283)

8 September 2017

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title

Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications (English)

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8 September 2017

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Pichia pastoris

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27905091%20AND%20SRC:MED&resulttype=core&format=json

6 June 2020

Hao Huang

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6 June 2020

Yunfeng Zhang

series ordinal

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6 June 2020

Guocheng Du

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27905091%20AND%20SRC:MED&resulttype=core&format=json

6 June 2020

Jian Chen

series ordinal

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6 June 2020

publication date

30 November 2016

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World Journal of Microbiology and Biotechnology

8 September 2017

published in

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6 June 2020

volume

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6 June 2020

issue

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6 June 2020

page(s)

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Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.

6 June 2020

cites work

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Construction of a novel Pichia pastoris strain for production of xanthophylls

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Rational diversification of a promoter providing fine-tuned expression and orthogonal regulation for synthetic biology

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Recombination within the yeast plasmid 2mu circle is site-specific

21 January 2018

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Heterologous protein expression in the methylotrophic yeast Pichia pastoris

21 January 2018

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Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

21 January 2018

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Safety evaluation of a lipase enzyme preparation, expressed in Pichia pastoris, intended for use in the degumming of edible vegetable oil.

21 January 2018

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Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review

21 January 2018

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Recombinant protein expression in Pichia pastoris

21 January 2018

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Expression in the yeast Pichia pastoris

21 January 2018

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Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications

21 January 2018

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Design of synthetic yeast promoters via tuning of nucleosome architecture.

21 January 2018

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Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast

21 January 2018

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Engineering dynamic pathway regulation using stress-response promoters.

21 January 2018

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Genome sequence of the recombinant protein production host Pichia pastoris

21 January 2018

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Prediction of proprotein convertase cleavage sites

21 January 2018

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Strains and molecular tools for recombinant protein production in Pichia pastoris

21 January 2018

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Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals

21 January 2018

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A new signal sequence for recombinant protein secretion in Pichia pastoris.

21 January 2018

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A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast

21 January 2018

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A new efficient gene disruption cassette for repeated use in budding yeast

21 January 2018

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Promoter library designed for fine-tuned gene expression in Pichia pastoris

21 January 2018

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Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain

21 January 2018

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https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

WoLF PSORT: protein localization predictor

21 January 2018

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https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Genome-scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

DATEL: A Scarless and Sequence-Independent DNA Assembly Method Using Thermostable Exonucleases and Ligase

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Combinatorial Evolution of Enzymes and Synthetic Pathways Using One-Step PCR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 January 2018

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https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

A combined transmembrane topology and signal peptide prediction method

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Enhanced production of leech hyaluronidase by optimizing secretion and cultivation in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Comparison of ADH3 promoter with commonly used promoters for recombinant protein production in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Recent advances in DNA assembly technologies

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Hydrophobin signal sequence mediates efficient secretion of recombinant proteins in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Production of recombinant human antithrombin by Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Expression of recombinant proteins in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Mxr1p, a key regulator of the methanol utilization pathway and peroxisomal genes in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Direct selection of Pichia pastoris expression strains using new G418 resistance vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Improvement of catalytic efficiency and thermostability of recombinant Streptomyces griseus trypsin by introducing artificial peptide.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Optimization of the production of Aspergillus niger α-glucosidase expressed in Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Comparative genomics and transcriptomics of Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Heterologous protein production using the Pichia pastoris expression system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Short Synthetic Terminators for Assembly of Transcription Units in Vitro and Stable Chromosomal Integration in Yeast S. cerevisiae

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

In-silico determination of Pichia pastoris signal peptides for extracellular recombinant protein production.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Endogenous signal peptides in recombinant protein production by Pichia pastoris: From in-silico analysis to fermentation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Cloning and disruption of the Pichia pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and their use as auxotrophic markers

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

The positive and negative cis-acting elements for methanol regulation in the Pichia pastoris AOX2 gene

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

SignalP 4.0: discriminating signal peptides from transmembrane regions

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Engineering of synthetic, stress-responsive yeast promoters

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

The development and characterization of synthetic minimal yeast promoters

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Rapid Selection Using G418 of High Copy Number Transformants of Pichia pastoris for High–level Foreign Gene Expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Microbials for the production of monoclonal antibodies and antibody fragments.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Expression of enzymes for the usage in food and feed industry with Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Regulation of Pichia pastoris promoters and its consequences for protein production

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Synthetic core promoters for Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

An upstream activation sequence controls the expression of AOX1 gene in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

A genome-wide activity assessment of terminator regions in Saccharomyces cerevisiae provides a ″terminatome″ toolbox

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

de novo design and synthesis of Candida antarctica lipase B gene and α-factor leads to high-level expression in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Recent advances on the GAP promoter derived expression system of Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS11274-016-2185-2

Yeast synthetic biology for the production of recombinant therapeutic proteins

21 January 2018

1 reference

12 December 2020

Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker

1 reference

12 December 2020

Comparison of alpha-factor preprosequence and a classical mammalian signal peptide for secretion of recombinant xylanase xynB from yeast Pichia pastoris.

1 reference

12 December 2020

Use of site-specific recombination to regenerate selectable markers

1 reference

12 December 2020

Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences

1 reference

12 December 2020

Functional expression of trypsin from Streptomyces griseus by Pichia pastoris

1 reference

12 December 2020

Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline β-mannanase in Pichia pastoris

1 reference

12 December 2020

Vector for pop-in/pop-out gene replacement in Pichia pastoris

1 reference

12 December 2020

FDA approves kallikrein inhibitor to treat hereditary angioedema

1 reference

12 December 2020

Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris

1 reference

12 December 2020

10.1007/S11274-016-2185-2

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27905091%20AND%20SRC:MED&resulttype=core&format=json

6 June 2020

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27905091%20AND%20SRC:MED&resulttype=core&format=json