Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors (Q34507124)

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English	Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors	scientific article

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scholarly article

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Europe PubMed Central

PubMed publication ID

2 August 2017

Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors (English)

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Europe PubMed Central

PubMed publication ID

2 August 2017

7

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Tom Taghon

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Europe PubMed Central

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2 August 2017

Massimo Pizzato

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Massimo Pizzato

8

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Jolien Vermeire

1

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Europe PubMed Central

PubMed publication ID

2 August 2017

Evelien Naessens

2

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Europe PubMed Central

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2 August 2017

Hanne Vanderstraeten

3

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Europe PubMed Central

PubMed publication ID

2 August 2017

Alessia Landi

4

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Europe PubMed Central

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2 August 2017

Veronica Iannucci

5

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Europe PubMed Central

PubMed publication ID

2 August 2017

Anouk Van Nuffel

6

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Europe PubMed Central

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2 August 2017

Bruno Verhasselt

9

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Europe PubMed Central

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2 August 2017

language of work or name

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publication date

5 December 2012

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Europe PubMed Central

PubMed publication ID

2 August 2017

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Europe PubMed Central

PubMed publication ID

2 August 2017

7

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Europe PubMed Central

PubMed publication ID

2 August 2017

12

1 reference

Europe PubMed Central

PubMed publication ID

2 August 2017

e50859

1 reference

Europe PubMed Central

PubMed publication ID

2 August 2017

copyright license

Creative Commons Attribution 4.0 International

5 December 2012

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April 2022 Public Data File from Crossref

copyright status

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Lentiviral Vectors: Their Molecular Design, Safety, and Use in Laboratory and Preclinical Research

1 reference

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7 July 2018

Rapid dissociation of HIV-1 from cultured cells severely limits infectivity assays, causes the inactivation ascribed to entry inhibitors, and masks the inherently high level of infectivity of virions

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Lentiviral vectors in clinical trials: Current status.

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7 July 2018

A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Nef-mediated enhancement of virion infectivity and stimulation of viral replication are fundamental properties of primate lentiviruses

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7 July 2018

Efficiency of human immunodeficiency virus type 1 postentry infection processes: evidence against disproportionate numbers of defective virions

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7 July 2018

A modified single-tube one-step product-enhanced reverse transcriptase (mSTOS-PERT) assay with heparin as DNA polymerase inhibitor for specific detection of RTase activity

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Comparison of lentiviral vector titration methods

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Real-time quantitative PCR for the design of lentiviral vector analytical assays

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7 July 2018

Human immunodeficiency virus Nef induces rapid internalization of the T-cell coreceptor CD8alphabeta

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7 July 2018

Factors influencing the titer and infectivity of lentiviral vectors.

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Relationships between infectious titer, capsid protein levels, and reverse transcriptase activities of diverse human immunodeficiency virus type 1 isolates

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events

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7 July 2018

Dynamics of HIV-1 recombination in its natural target cells

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7 July 2018

Signaling but not trafficking function of HIV-1 protein Nef is essential for Nef-induced defects in human intrathymic T-cell development

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Real-Time Quantitative Reverse Transcriptase-Polymerase Chain Reaction as a Method for Determining Lentiviral Vector Titers and Measuring Transgene Expression

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Identification and characterization of avian retroviruses in chicken embryo-derived yellow fever vaccines: investigation of transmission to vaccine recipients

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7 July 2018

Comparing the ex vivo fitness of CCR5-tropic human immunodeficiency virus type 1 isolates of subtypes B and C.

1 reference

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7 July 2018

Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods

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7 July 2018

Gene transduction efficiency in cells of different species by HIV and EIAV vectors

1 reference

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7 July 2018

Evaluation of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus in mAb cell-culture

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Long-term follow-up failed to detect in vitro transmission of full-length porcine endogenous retroviruses from specific pathogen-free pig islets to human cells

1 reference

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7 July 2018

Characterization of endogenous avian leukosis viruses in chicken embryonic fibroblast substrates used in production of measles and mumps vaccines

1 reference

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7 July 2018

No evidence of infectious retroviruses in measles virus vaccines produced in chicken embryo cell cultures

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods

1 reference

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7 July 2018

Development of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 and performance of an improved AMPLICOR HIV-1 MONITOR test with isolates of diverse subtypes

1 reference

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7 July 2018

Improved Mg2+-based reverse transcriptase assay for detection of primate retroviruses

1 reference

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7 July 2018

One-step fluorescent probe product-enhanced reverse transcriptase assay.

1 reference

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7 July 2018

Development of an improved product enhanced reverse transcriptase assay

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3515444

7 July 2018

Highly sensitive qualitative and quantitative detection of reverse transcriptase activity: optimization, validation, and comparative analysis with other detection systems

1 reference

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7 July 2018

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector

1 reference

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7 July 2018

An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions

1 reference

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7 July 2018

HIV-1 Reverse Transcription A Termination Step at the Center of the Genome

1 reference

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7 July 2018

The ratio of defective HIV-1 particles to replication-competent infectious virions

1 reference

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7 July 2018

Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement

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7 July 2018

Evaluation of different RT enzyme standards for quantitation of retroviruses using the single-tube fluorescent product-enhanced reverse transcriptase assay.

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

21 January 2018

Detectionof reverse transcriptase activity in live attenuated virus vaccines

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

21 January 2018

Use of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus levels in mAb cell-culture and downstream processing

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

21 January 2018

Evidence of porcine endogenous retroviruses in porcine factor VIII and evaluation of transmission to recipients with hemophilia

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

21 January 2018

The reverse transcriptase activity in cell-free medium of chicken embryo fibroblast cultures is not associated with a replication-competent retrovirus

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

21 January 2018

Efficient magnetic bead-based separation of HIV-1-infected cells using an improved reporter virus system reveals that p53 up-regulation occurs exclusively in the virus-expressing cell population

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

21 January 2018

Cell surface CD4 interferes with the infectivity of HIV-1 particles released from T cells

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https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0050859

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Human immunodeficiency virus nef gene expression affects generation and function of human T cells, but not dendritic cells

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27 October 2018

Measurement of human immunodeficiency virus type 1 plasma virus load based on reverse transcriptase (RT) activity: evidence of variabilities in levels of virion-associated RT.

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Magselectofection: an integrated method of nanomagnetic separation and genetic modification of target cells

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Adaptation of the fluorogenic 5'-nuclease chemistry to a PCR-based reverse transcriptase assay

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https://pubmed.ncbi.nlm.nih.gov/23227216

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Analysis of a coded panel of licensed vaccines by polymerase chain reaction-based reverse transcriptase assays: a collaborative study [seecomments]

1 reference

https://pubmed.ncbi.nlm.nih.gov/23227216

12 December 2020

based on heuristic

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A new method for measuring reverse transcriptase activity by ELISA

1 reference

https://pubmed.ncbi.nlm.nih.gov/23227216

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Superiority of infectivity-based over particle-based methods for quantitation of drug resistant HIV-1 as inocula for cell cultures

1 reference

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Single-tube fluorescent product-enhanced reverse transcriptase assay with Ampliwax (STF-PERT) for retrovirus quantitation

1 reference

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10.1371/JOURNAL.PONE.0050859

1 reference

Europe PubMed Central

PubMed publication ID

2 August 2017

2012PLoSO...750859V

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PMC publication ID

1 reference

Europe PubMed Central

PubMed publication ID

2 August 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

2 August 2017

ResearchGate publication ID

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