Directed evolution provides insight into conformational substrate sampling by SrtA. (Q38599712)

4 October 2017

Directed evolution provides insight into conformational substrate sampling by SrtA. (English)

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4 October 2017

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11 June 2020

Muna Suliman

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11 June 2020

Vishaka Santosh

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11 June 2020

Tom C M Seegar

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11 June 2020

Annamarie C Dalton

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11 June 2020

Kathryn M Schultz

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11 June 2020

Candice S Klug

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11 June 2020

language of work or name

English

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publication date

31 August 2017

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4 October 2017

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11 June 2020

volume

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11 June 2020

issue

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11 June 2020

page(s)

e0184271

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Creative Commons Attribution 4.0 International

11 June 2020

start time

31 August 2017

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April 2022 Public Data File from Crossref

copyrighted

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cites work

Discerning the catalytic mechanism of Staphylococcus aureus sortase A with QM/MM free energy calculations

1 reference

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23 June 2018

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23 June 2018

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Structural Basis for Group B Streptococcus Pilus 1 Sortases C Regulation and Specificity

23 June 2018

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23 June 2018

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Protein ligation in living cells using sortase

23 June 2018

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The Crystal Structure Analysis of Group B Streptococcus Sortase C1: A Model for the “Lid” Movement upon Substrate Binding

23 June 2018

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Sortase enzymes in Gram-positive bacteria

23 June 2018

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Directed evolution of sortase A mutants with altered substrate selectivity profiles

23 June 2018

1 reference

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Computer modeling of nitroxide spin labels on proteins

23 June 2018

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A general strategy for the evolution of bond-forming enzymes using yeast display

23 June 2018

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Architects at the bacterial surface — sortases and the assembly of pili with isopeptide bonds

23 June 2018

1 reference

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A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.

23 June 2018

1 reference

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Two crystal structures of pneumococcal pilus sortase C provide novel insights into catalysis and substrate specificity

23 June 2018

1 reference

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Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation

23 June 2018

1 reference

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The structure of the Staphylococcus aureus sortase-substrate complex reveals how the universally conserved LPXTG sorting signal is recognized

23 June 2018

1 reference

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A straight path to circular proteins

23 June 2018

1 reference

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Sortase-mediated ligation: a gift from Gram-positive bacteria to protein engineering.

23 June 2018

1 reference

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Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligation

23 June 2018

1 reference

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Sortagging: a versatile method for protein labeling

23 June 2018

1 reference

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Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins

23 June 2018

1 reference

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Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site

23 June 2018

1 reference

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Crystal structures of Staphylococcus aureus sortase A and its substrate complex.

23 June 2018

1 reference

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Sortase-mediated protein ligation: a new method for protein engineering

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

The sortase SrtA of Listeria monocytogenes is involved in processing of internalin and in virulence

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Inactivation of the srtA gene in Streptococcus gordonii inhibits cell wall anchoring of surface proteins and decreases in vitro and in vivo adhesion

23 June 2018

1 reference

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Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections

23 June 2018

1 reference

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Sortase, a universal target for therapeutic agents against gram-positive bacteria?

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

23 June 2018

1 reference

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Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid

23 June 2018

1 reference

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Site-specific biotinylation of purified proteins using BirA.

23 June 2018

1 reference

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The binding mechanism, multiple binding modes, and allosteric regulation of Staphylococcus aureus Sortase A probed by molecular dynamics simulations

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Protein-fragment complementation assays for large-scale analysis, functional dissection and dynamic studies of protein-protein interactions in living cells

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Enzymatic ligation of peptides, peptide nucleic acids and proteins by means of sortase A.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Detection of protein-protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Sortase A as a novel molecular "stapler" for sequence-specific protein conjugation

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Staphylococcus aureus sortase transpeptidase SrtA: insight into the kinetic mechanism and evidence for a reverse protonation catalytic mechanism

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Kinetic Mechanism of Staphylococcus aureus Sortase SrtA

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Cysteine 184 and histidine 120 of sortase form a thiolate-imidazolium ion pair for catalysis

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5578623

Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site

31 August 2018

1 reference

Crossref

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0184271

Dead-Time Free Measurement of Dipole–Dipole Interactions between Electron Spins

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0184271

Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/28859178

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1371/JOURNAL.PONE.0184271

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:28859178%20AND%20SRC:MED&resulttype=core&format=json

11 June 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:28859178%20AND%20SRC:MED&resulttype=core&format=json

11 June 2020

PubMed publication ID

28859178

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:28859178%20AND%20SRC:MED&resulttype=core&format=json