LightCycler technology in molecular diagnostics (Q37386543)

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scientific article published on 05 February 2009

Language	Label	Description	Also known as
English	LightCycler technology in molecular diagnostics	scientific article published on 05 February 2009

Statements

scholarly article

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

LightCycler technology in molecular diagnostics (English)

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

0 references

author name string

Elaine Lyon

1

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

Carl T Wittwer

2

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

publication date

5 February 2009

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

The Journal of Molecular Diagnostics

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

11

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

2

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

93-101

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

1 reference

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6 September 2017

Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Design and application of noncontinuously binding probes used for haplotyping and genotyping

1 reference

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6 September 2017

Unlabeled probes for the detection and typing of herpes simplex virus

1 reference

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6 September 2017

RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Molecular diagnosis of Prader-Willi and Angelman syndromes by methylation-specific melting analysis and methylation-specific multiplex ligation-dependent probe amplification

1 reference

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6 September 2017

Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes

1 reference

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6 September 2017

Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Real-time PCR in clinical microbiology: applications for routine laboratory testing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Factor V null mutation affecting the Roche LightCycler factor V Leiden assay

1 reference

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6 September 2017

Direct molecular haplotyping by melting curve analysis of hybridization probes: beta 2-adrenergic receptor haplotypes as an example

1 reference

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6 September 2017

Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: a novel strategy for PCR detection and genotyping.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

High-resolution genotyping by amplicon melting analysis using LCGreen

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Rapid-cycle PCR and fluorimetry for detection of mycobacteria

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Mutation detection using fluorescent hybridization probes and melting curve analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Fluorescein-labeled oligonucleotides for real-time pcr: using the inherent quenching of deoxyguanosine nucleotides

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Product differentiation by analysis of DNA melting curves during the polymerase chain reaction

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Real time quantitative PCR

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Rapid cycle DNA amplification: time and temperature optimization

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Confirmation of single exon deletions in MLH1 and MSH2 using quantitative polymerase chain reaction

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

6 September 2017

Warfarin dose and INR related to genotypes of CYP2C9 and VKORC1 in patients with myocardial infarction

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

14 September 2017

Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

14 September 2017

Inter-laboratory comparison of chronic myeloid leukemia minimal residual disease monitoring: summary and recommendations

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

14 September 2017

Single-tube genotyping without oligonucleotide probes.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

14 September 2017

Rapid and sensitive detection of BRCA1/2 mutations in a diagnostic setting: comparison of two high-resolution melting platforms

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

26 June 2018

Agreement in breast cancer classification between microarray and quantitative reverse transcription PCR from fresh-frozen and formalin-fixed, paraffin-embedded tissues

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

26 June 2018

Comparison of two quantitative polymerase chain reaction methods for detecting HER2/neu amplification

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

26 June 2018

Genotyping of eight thiopurine methyltransferase mutations: three-color multiplexing, "two-color/shared" anchor, and fluorescence-quenching hybridization probe assays based on thermodynamic nearest-neighbor probe design.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

26 June 2018

Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

26 June 2018

Snapback primer genotyping with saturating DNA dye and melting analysis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

High-resolution melting analysis for rapid screening of BRCA1 and BRCA2 Spanish mutations.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

High-throughput amplicon scanning of the TP53 gene in breast cancer using high-resolution fluorescent melting curve analyses and automatic mutation calling.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Randomized trial of genotype-guided versus standard warfarin dosing in patients initiating oral anticoagulation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Detection of factor VIII gene mutations by high-resolution melting analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Mutation scanning the GJB1 gene with high-resolution melting analysis: implications for mutation scanning of genes for Charcot-Marie-Tooth disease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Discovering rare variants by use of melting temperature shifts seen in melting curve analysis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Human malignant melanoma: detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Application of rapid-cycle real-time polymerase chain reaction for diagnostic testing in the clinical microbiology laboratory

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Detection of a novel point mutation of the prothrombin gene at position 20209.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Simultaneous detection of methylenetetrahydrofolate reductase gene polymorphisms, C677T and A1298C, by melting curve analysis with LightCycler

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Limitations of genotyping based on amplicon melting temperature.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

In-tube DNA methylation profiling by fluorescence melting curve analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

LightCycler technology for the quantitation of bcr/abl fusion transcripts.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2665858

2 September 2018

Simultaneous detection of C282Y and H63D hemochromatosis mutations by dual-color probes

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Atypical Melting Curve Resulting from Genetic Variation in the 3' Untranslated Region at Position 20218 in the Prothrombin Gene Analyzed with the LightCycler Factor II (Prothrombin) G20210A Assay

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Continuous fluorescence monitoring of rapid cycle DNA amplification

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Detection and Identification of Base Alterations Within the Region of Factor V Leiden by Fluorescent Melting Curves

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Masking selected sequence variation by incorporating mismatches into melting analysis probes

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Quantification of HER2/neu gene amplification by competitive pcr using fluorescent melting curve analysis

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis.

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Epidermal growth factor receptor mutation detection using high-resolution melting analysis predicts outcomes in patients with advanced non small cell lung cancer treated with gefitinib

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Minimizing the time required for DNA amplification by efficient heat transfer to small samples

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Functional analysis of two prothrombin 3'-untranslated region variants: the C20209T variant, mainly found among African-Americans, and the C20209A variant

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Allelic discrimination using fluorogenic probes and the 5' nuclease assay

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Influence of DNA target melting behavior on real-time PCR quantification

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

High-resolution melting amplicon analysis as a method to detect c-kit and platelet-derived growth factor receptor alpha activating mutations in gastrointestinal stromal tumors

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using fluorescently labeled probes

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifying common genetic variants by high-resolution melting

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Detection of exon deletions within an entire gene (CFTR) by relative quantification on the LightCycler

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid detection of aneuploidy (trisomy 21) by allele quantification combined with melting curves analysis of single-nucleotide polymorphism loci

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer

1 reference

https://pubmed.ncbi.nlm.nih.gov/19196999

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.2353/JMOLDX.2009.080094

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

20 August 2017

ResearchGate publication ID

0 references

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