An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms (Q83227163)

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scientific article published on 01 November 2019

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English	An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms	scientific article published on 01 November 2019

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19 April 2020

An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms (English)

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19 April 2020

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19 April 2020

Karen L Schulze

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19 April 2020

Allan C Spradling

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19 April 2020

Stephanie E Mohr

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19 April 2020

Norbert Perrimon

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19 April 2020

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Gabriel Amador

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Jorge García-Marqués

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Jorge Garcia-Marques

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Jonathan Zirin

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19 April 2020

Shannon Marie Knight

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19 April 2020

Donghui Yang-Zhou

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19 April 2020

Hyunglok Chung

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Zhongyuan Zuo

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Liwen Ma

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19 April 2020

Yuchun He

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19 April 2020

Wen-Wen Lin

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19 April 2020

Ying Fang

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19 April 2020

Ming Ge

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19 April 2020

Shinya Yamamoto

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19 April 2020

Yanhui Hu

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19 April 2020

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1 November 2019

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19 April 2020

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19 April 2020

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19 April 2020

describes a project that uses

object named as

CellProfiler

1 reference

CZ Software Mentions: A large dataset of software mentions in the biomedical literature

0 references

polo encodes a protein kinase homolog required for mitosis in Drosophila

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction

1 reference

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12 December 2020

based on heuristic

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Targeted gene expression as a means of altering cell fates and generating dominant phenotypes

1 reference

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12 December 2020

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Type I repressors of P element mobility.

1 reference

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12 December 2020

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The DNA binding and activation domains of Gal4p are sufficient for conveying its regulatory signals

1 reference

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12 December 2020

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Imaging cells in the developing nervous system with retrovirus expressing modified green fluorescent protein

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Characterization of the interaction of lambda exonuclease with the ends of DNA.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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In vivo localisation of the mitotic POLO kinase shows a highly dynamic association with the mitotic apparatus during early embryogenesis in Drosophila

1 reference

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12 December 2020

based on heuristic

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Gene targeting by homologous recombination in Drosophila

1 reference

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12 December 2020

based on heuristic

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Highly sensitive, fluorescent transformation marker for Drosophila transgenesis.

1 reference

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Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

1 reference

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Engineering and characterization of a superfolder green fluorescent protein

1 reference

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based on heuristic

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Evaluation of a novel short polyadenylation signal as an alternative to the SV40 polyadenylation signal.

1 reference

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12 December 2020

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Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange

1 reference

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12 December 2020

based on heuristic

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Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases.

1 reference

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12 December 2020

based on heuristic

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The transcriptional diversity of 25 Drosophila cell lines

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Fluorescent fusion protein knockout mediated by anti-GFP nanobody

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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A GAL4-driver line resource for Drosophila neurobiology

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.

1 reference

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12 December 2020

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Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

1 reference

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Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

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Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans

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A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila.

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FlyBase: introduction of the Drosophila melanogaster Release 6 reference genome assembly and large-scale migration of genome annotations

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Gene-specific cell labeling using MiMIC transposons.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

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based on heuristic

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Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

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based on heuristic

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A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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A genetic toolkit for tagging intronic MiMIC containing genes

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High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.

1 reference

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CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

1 reference

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Morgan's legacy: fruit flies and the functional annotation of conserved genes.

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Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.

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WAC Regulates mTOR Activity by Acting as an Adaptor for the TTT and Pontin/Reptin Complexes

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A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells

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A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function

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CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

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12 December 2020

based on heuristic

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In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

1 reference

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A Syndromic Neurodevelopmental Disorder Caused by De Novo Variants in EBF3

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Loss of Nardilysin, a Mitochondrial Co-chaperone for α-Ketoglutarate Dehydrogenase, Promotes mTORC1 Activation and Neurodegeneration

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based on heuristic

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FlpStop, a tool for conditional gene control in Drosophila.

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based on heuristic

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Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.

1 reference

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Synthetic Lethality Screens Using RNAi in Combination with CRISPR-based Knockout in Drosophila Cells.

1 reference

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based on heuristic

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CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms

1 reference

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based on heuristic

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A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells.

1 reference

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Gene Tagging Strategies To Assess Protein Expression, Localization, and Function in Drosophila.

1 reference

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Genetic strategies to tackle neurological diseases in fruit flies.

1 reference

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Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks

1 reference

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12 December 2020

based on heuristic

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Advances in Engineering the Fly Genome with the CRISPR-Cas System.

1 reference

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A kinase-dependent feedforward loop affects CREBB stability and long term memory formation.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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A gene-specific T2A-GAL4 library for Drosophila.

1 reference

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12 December 2020

based on heuristic

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Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells

1 reference

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12 December 2020

based on heuristic

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Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in cells

1 reference

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12 December 2020

based on heuristic

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An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

FlyBase 2.0: the next generation

1 reference

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12 December 2020

based on heuristic

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Unlimited Genetic Switches for Cell-Type-Specific Manipulation

1 reference

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12 December 2020

based on heuristic

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Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids

1 reference

https://pubmed.ncbi.nlm.nih.gov/31674908

12 December 2020

based on heuristic

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10.7554/ELIFE.51539

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19 April 2020

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19 April 2020

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19 April 2020

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