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Inhibition of merozoite invasion and transient de-sequestration by sevuparin in humans with Plasmodium falciparum malaria

PLoS One. 2017 Dec 15;12(12):e0188754. doi: 10.1371/journal.pone.0188754. eCollection 2017.

Abstract

Severe malaria: Even with the best available treatment, the mortality from severe Plasmodium falciparum malaria remains high. Typical features at death are high parasite loads and obstructed micro- vasculature. Infected erythrocytes (IE) containing mature parasites bind to the host receptor heparan sulfate, which is also an important receptor for merozoite invasion. To block merozoite invasion has not previously been proposed as an adjunctive therapeutic approach but it may preclude the early expansion of an infection that else leads to exacerbated sequestration and death.

Sevuparin in phase i study: The drug sevuparin was developed from heparin because heparan sulfate and heparin are nearly identical, so the rationale was that sevuparin would act as a decoy receptor during malaria infection. A phase I study was performed in healthy male volunteers and sevuparin was found safe and well tolerated.

Sevuparin in phase i/ii clinical study: A phase I/II clinical study was performed in which sevuparin was administered via short intravenous infusions to malaria patients with uncomplicated malaria who were also receiving atovaquone/proguanil treatment. This was a Phase I/II, randomized, open label, active control, parallel assignment study. Sevuparin was safe and well tolerated in the malaria patients. The mean relative numbers of ring-stage IEs decreased after a single sevuparin infusion and mature parasite IEs appeared transiently in the circulation. The effects observed on numbers of merozoites and throphozoites in the circulation, were detected already one hour after the first sevuparin injection. Here we report the development of a candidate drug named sevuparin that both blocks merozoite invasion and transiently de-sequesters IE in humans with P. falciparum malaria.

Trial registration: ClinicalTrials.gov NCT01442168.

Publication types

  • Clinical Trial, Phase I
  • Clinical Trial, Phase II
  • Multicenter Study
  • Randomized Controlled Trial

MeSH terms

  • Administration, Oral
  • Adolescent
  • Adult
  • Aged
  • Antimalarials / blood
  • Antimalarials / pharmacokinetics
  • Antimalarials / pharmacology*
  • Area Under Curve
  • Atovaquone / blood
  • Atovaquone / pharmacokinetics
  • Atovaquone / pharmacology*
  • Binding, Competitive
  • Drug Administration Schedule
  • Drug Combinations
  • Drug Therapy, Combination
  • Erythrocytes / drug effects
  • Erythrocytes / parasitology
  • Female
  • Heparin / analogs & derivatives*
  • Heparin / blood
  • Heparin / pharmacokinetics
  • Heparin / pharmacology
  • Heparitin Sulfate / chemistry
  • Heparitin Sulfate / metabolism
  • Humans
  • Infusions, Intravenous
  • Malaria, Falciparum / blood
  • Malaria, Falciparum / drug therapy*
  • Malaria, Falciparum / parasitology
  • Male
  • Merozoites / drug effects*
  • Merozoites / physiology
  • Middle Aged
  • Parasite Load
  • Parasitemia / blood
  • Parasitemia / drug therapy*
  • Parasitemia / parasitology
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / physiology
  • Proguanil / blood
  • Proguanil / pharmacokinetics
  • Proguanil / pharmacology*
  • Severity of Illness Index

Substances

  • Antimalarials
  • Drug Combinations
  • atovaquone, proguanil drug combination
  • sevuparin
  • Heparin
  • Heparitin Sulfate
  • Proguanil
  • Atovaquone

Associated data

  • ClinicalTrials.gov/NCT01442168