A CRISPR-based assay both recognizes and amplifies target mRNA, achieving sub-attomolar sensitivity with single-nucleotide resolution. This method enables the detection of low-abundance mRNA in extracellular vesicles, providing clinically relevant information for precision oncology.
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This is a summary of: Song, J. et al. Amplifying mutational profiling of extracellular vesicle mRNA with SCOPE. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02426-6 (2024).
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A CRISPR-based method for detecting mRNA in extracellular vesicles. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02440-8
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DOI: https://doi.org/10.1038/s41587-024-02440-8