Supplementary Figure 8: Csn induces apoptosis in TRPV1–expressing cells exposed to high Csn levels but does not ablate sensory neurons that do not express TRPV1.

(a–d) Representative images showing that TUNEL labeling was only observed in TRPV1⁺ neurons exposed to 10 µM Csn. Starting at 24 h.p.f., larvae were exposed to Csn for 6 hours and then processed for TUNEL staining. (e) Mean ± s.e.m. percentage of TRPV1⁺ Rohon–Beard neurons that are TUNEL positive in a 340 µm region of spinal cord that typically contained ~10 Rohon–Beard neurons. Seven embryos were analyzed for each condition. (f,g) Representative images of Et(e1b:GAL4VP16)s1102t;Tg(14xUAS:EGFP-Aequorin) embryos incubated in vehicle control or 10 μM Csn starting at 28 h.p.f. EGFP fluorescence is unaffected by Csn treatment. (h) Mean ± s.e.m. EGFP fluorescence intensity for the conditions shown in (f,g). Three embryos were analyzed for each condition. The yellow boxes in (f–f”) indicate the regions of interest used to quantify fluorescence at each time point. Scale bars, 50 μm (a–d) and 100 μm (f,g).