| Entry |
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| Name |
23S rRNA (guanine745-N1)-methyltransferase;
Rlma(I);
Rlma1;
23S rRNA m1G745 methyltransferase;
YebH;
RlmAI methyltransferase;
ribosomal RNA(m1G)-methylase (ambiguous);
rRNA(m1G)methylase (ambiguous);
RrmA (ambiguous);
23S rRNA:m1G745 methyltransferase
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
S-adenosyl-L-methionine:23S rRNA (guanine745-N1)-methyltransferase
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| Reaction(IUBMB) |
S-adenosyl-L-methionine + guanine745 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA [RN: R07233]
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| Reaction(KEGG) |
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| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
guanine745 in 23S rRNA
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| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
N1-methylguanine745 in 23S rRNA
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| Comment |
The enzyme specifically methylates guanine745 at N1 in 23S rRNA.
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| History |
EC 2.1.1.187 created 1976 as EC 2.1.1.51, part transferred 2010 to EC 2.1.1.187
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| Orthology |
| K00563 | 23S rRNA (guanine745-N1)-methyltransferase |
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| Genes |
| ECOO: | ECRM13514_2327(rrmA) |
| ECOH: | ECRM13516_2230(rrmA) |
| ELR: | ECO55CA74_10925(rrmA) |
| ECW: | EcE24377A_2049(rrmA) |
| ECOA: | APECO78_12910(rrmA) |
| EDJ: | ECDH1ME8569_1767(rrmA) |
| ECOI: | ECOPMV1_01914(rlmA) |
| SEM: | STMDT12_C18550(rrmA) |
| SETC: | CFSAN001921_07915(rrmA) |
| SEEN: | SE451236_15115(rrmA) |
| SENH: | CFSAN002069_22665(rrmA) |
| SEEH: | SEEH1578_18430(rrmA) |
| SENJ: | CFSAN001992_02300(rrmA) |
| SEEC: | CFSAN002050_15650(rrmA) |
| SEEB: | SEEB0189_010320(rrmA) |
| SENC: | SEET0819_15920(rrmA) |
| REE: | electrica_01906(rlmA) |
| RTG: | NCTC13098_02774(rlmA) |
| CLAP: | NCTC11466_01810(rlmA) |
| KIE: | NCTC12125_00979(rlmA) |
| AHN: | NCTC12129_02025(rrmA) |
| PSHI: | SAMEA2665130_0920(rlmA) |
| MPHG: | MPHASIOC01_001498(rlmA) |
| YPG: | YpAngola_A1660(rrmA) |
| YPI: | YpsIP31758_2376(rrmA) |
| SMW: | SMWW4_v1c28370(rlmA) |
| SERS: | SERRSCBI_13355(rrmA) |
| SFJ: | SAMEA4384070_2863(rlmA) |
| SOF: | NCTC11214_03962(rlmA) |
| SSAR: | SSARUM_002748(rlmA) |
| PAQU: | DMB82_0011405(rlmA) |
| PARL: | PEC302110_19160(rlmA) |
| DIC: | Dpoa569_001827(rlmA) |
| TPTY: | NCTC11468_02088(rlmA) |
| PHEI: | NCTC12003_01840(rlmA) |
| PRJ: | NCTC6933_02082(rlmA) |
| LRI: | NCTC12151_01886(rlmA) |
| VAU: | VANGNB10_cI0934c(rrmA) |
| VPL: | SA104470976_01705(rrmA) |
| VIB: | VspSTUT11_08020(rrmA) |
| VIS: | VspSTUT16_09010(rrmA) |
| PAEB: | NCGM1900_1432(rrmA) |
| PAEU: | BN889_01239(rrmA_1) |
| PPUD: | DW66_1124 DW66_1458 |
| PPRC: | PFLCHA0_c11840(rlmA) |
| PMUD: | NCTC8068_01169(rlmA) |
| PFW: | PF1751_v1c12050(rrmA) |
| PPUU: | PputUW4_01075(rlmA) |
| PTAE: | NCTC10697_03470(rlmA) |
| PSYC: | DABAL43B_2676(rrmA) |
| MCAT: | MC25239_01596(rlmA) |
| MCUN: | NCTC10297_00267(rlmA) |
| SON: | SO_1294 SO_2788(rlmA) |
| SBM: | Shew185_1197 Shew185_1704 |
| SBN: | Sbal195_1230 Sbal195_1747 |
| SBP: | Sbal223_2660 Sbal223_3160 |
| SBT: | Sbal678_1260 Sbal678_1786 |
| SBS: | Sbal117_1253 Sbal117_1820 |
| SBB: | Sbal175_2633 Sbal175_3130 |
| SPC: | Sputcn32_1113 Sputcn32_1571 |
| SHP: | Sput200_1118 Sput200_1588 |
| SHE: | Shewmr4_2408 Shewmr4_2899 |
| SHM: | Shewmr7_2478 Shewmr7_2981 |
| SHN: | Shewana3_2570 Shewana3_3078 |
| SHW: | Sputw3181_2451 Sputw3181_3051 |
| SVO: | SVI_0887 SVI_2531(rrmA) |
| SHF: | CEQ32_02500 CEQ32_09480 |
| SJA: | SJ2017_1190 SJ2017_2410 |
| SPSW: | Sps_01351 Sps_02544 |
| SBJ: | CF168_05460 CF168_08045 |
| SMAV: | CFF01_11280 CFF01_14385 |
| SHEW: | CKQ84_01805 CKQ84_22815 |
| SALG: | BS332_01375 BS332_16945 |
| SLJ: | EGC82_13295 EGC82_17115 |
| SMAI: | EXU30_01220 EXU30_04420 |
| SPOL: | FH971_04850 FH971_08350(rlmA) |
| SBK: | SHEWBE_1575 SHEWBE_2425(rrmA) |
| SKH: | STH12_00360(rlmA_1) STH12_01660(rlmA_2) |
| SAES: | HBH39_07460(rlmA) HBH39_12930 |
| SLIT: | JQC75_03930 JQC75_10080(rlmA) |
| SCAA: | TUM17387_10850 TUM17387_22170(rlmA) |
| SPSH: | FM037_16360(rlmA) FM037_22185 |
| SEUR: | FM038_005425 FM038_010185(rlmA) |
| SCYP: | JYB88_03765 JYB88_07715(rlmA) |
| SSEM: | JYB85_03960 JYB85_07890(rlmA) |
| SGLA: | FJ709_11050(rlmA) FJ709_14550 |
| SINV: | K8B83_14610(rlmA) K8B83_18450 |
| SXM: | MKD32_08365(rlmA) MKD32_15080 |
| SVM: | KDH10_001260(rlmA) KDH10_004065 |
| SHEJ: | MZ182_05285 MZ182_07720(rlmA) |
| SSEH: | N7V09_07885 N7V09_10440(rlmA) |
| SCHK: | GII14_12500(rlmA) GII14_16230 |
| SACH: | K0H61_10890(rlmA) K0H61_12965 |
| SAEG: | K0H80_05010 K0H80_08215(rlmA) |
| SDEO: | D0436_13710(rlmA) D0436_16305 |
| SMES: | K0I73_11135(rlmA) K0I73_14220 |
| SHAO: | K0H81_11785(rlmA) K0H81_15095 |
| SZH: | K0H63_04560 K0H63_11085(rlmA) |
| SSPA: | K0I31_05035 K0I31_08365(rlmA) |
| SHNS: | K0J45_05025 K0J45_08190(rlmA) |
| SRHS: | K0I63_05100 K0I63_08260(rlmA) |
| SPSJ: | K0I62_11775(rlmA) K0I62_15040 |
| SMAY: | K0H60_12985(rlmA) K0H60_15405 |
| SOG: | RA178_13915(rlmA) RA178_16380 |
| SPSR: | EGC80_10005 EGC80_18305 |
| SHEA: | GUY17_08845(rlmA) GUY17_15975 |
| SHEL: | JM642_08265(rlmA) JM642_14800 |
| SHEH: | ABIS04_04570 ABIS04_07955(rlmA) |
| HAXI: | HAALTHF_51710n(rrmA) |
| ALCA: | ASALC70_03072(rlmA) |
| AEL: | NCTC12917_03385(rrmA) |
| CDIZ: | CEDIAZO_01552(rlmA) |
| PLG: | NCTC10937_01455(rlmA) |
| THAG: | CKCBHOJB_00313(rlmA) |
| OBG: | Verru16b_03548(rlmA) |
| BAZ: | BAMTA208_19705(yxjB) |
| BEO: | BEH_24335 BEH_26101 BEH_26115 |
| BLEN: | NCTC4824_00395(rlmA) |
| LGZ: | NCTC10812_00950(rlmA) |
| GMO: | NCTC11323_01609(rlmA) |
| GHA: | NCTC10459_01643(rlmA) |
| LLK: | LLKF_1418 LLKF_2179(yuhD) |
| LLS: | lilo_1301 lilo_1982(yuhD) |
| LLD: | P620_07610 P620_11510 |
| LLX: | NCDO2118_1380 NCDO2118_2102 |
| LLJ: | LG36_1057 LG36_1932(yuhD) |
| LLM: | llmg_1124 llmg_2246(rrmA) |
| LLN: | LLNZ_05810 LLNZ_11585 |
| LLI: | uc509_1342 uc509_1958(rrmA) |
| LACT: | D7I46_03270 D7I46_07945 |
| LACK: | FLP15_00540 FLP15_08270 |
| LTI: | JW886_06275 JW886_09340 |
| LACN: | QJV49_01515 QJV49_05705 |
| SVB: | NCTC12167_01778(rlmA1) |
| SPSU: | NCTC13786_01010(rlmA) |
| SPOC: | NCTC10925_00204(rlmA) |
| SAUP: | NCTC3168_00570(rrmA) |
| SVF: | NCTC3166_00203(rrmA) |
| SMIE: | NCTC11169_00182(rrmA) |
| LOR: | AYI71_03560 AYI71_06120 |
| LHO: | LOOC260_113920(rrmA) |
| EFU: | HMPREF0351_12412(rrmA) |
| ECEC: | NCTC12421_00611(rlmA) |
| CGEL: | psyc5s11_17140(rrmA) |
| PFAA: | MM59RIKEN_08470(rlmA) |
| VFA: | MM35RIKEN_08970(rlmA) |
| VCOP: | MM50RIKEN_02610(rlmA) |
| PHX: | KGNDJEFE_01833(rlmA_1) KGNDJEFE_02274(rlmA_2) |
| AARG: | Aargi30884_07120(rrmA) |
| ABSI: | A9CBEGH2_08960(rrmA) |
| FIT: | Fi14EGH31_27040(rrmA) |
| CDIP: | ERS451417_00891(rrmA) |
| CUN: | Cul210932_0872(rrmA) |
| CUQ: | Cul210931_0834(rrmA) |
| CUJ: | CUL131002_0850(rrmA) |
| CMIN: | NCTC10288_01546(rrmA) |
| CPEG: | CPELA_06960(rlmAII) |
| CRL: | NCTC7448_01653(RrmA) |
| CCHO: | CCHOA_04235(rlmAII) |
| CPSO: | CPPEL_07185(rlmAII) |
| CCYS: | SAMEA4530656_1431(rrmA) |
| CCOE: | CETAM_05020(rlmAII) |
| CMAS: | CMASS_04090(rlmAII) |
| CHEI: | CHEID_07050(rlmAII) |
| CIHU: | CIHUM_04410(rlmAII) |
| CAQM: | CAQUA_07320(rlmAII) |
| CCAW: | CCANI_05170(rlmAII) |
| CAUI: | CAURIS_04265(rlmAII) |
| CFAC: | CFAEC_05050(rlmAII1) CFAEC_13900(rlmAII2) |
| CFOU: | CFOUR_04345(rlmAII) |
| CFEU: | CFELI_04820(rlmAII) |
| CAUS: | CAURIC_07500(rlmAII) |
| CATR: | CATRI_04800(rlmAII) |
| CCOU: | CCONF_04665(rlmAII) |
| CAFE: | CAFEL_04290(rlmAII) |
| CGOI: | CGOTT_04450(rlmAII) |
| CBOV: | CBOVI_03740(rlmAII) |
| NFR: | ERS450000_05205(rlmA) |
| NAD: | NCTC11293_06042(rlmA) |
| RCR: | NCTC10994_01454(rlmA) |
| SMA: | SAVERM_4467 SAVERM_77 SAVERM_80 |
| SRW: | TUE45_pSRc_0437(rlmAII) |
| STUI: | GCM10017668_36510(rrmA) |
| SHUN: | DWB77_07504(rlmAII) |
| AMAU: | DSM26151_14430(rlmAII) |
| AAGI: | NCTC2676_1_01382(rlmA) |
| DCO: | SAMEA4475696_1634(rlmA) |
| CGRN: | 4412665_01081(rlmA) |
| PAUS: | NCTC13651_01798(rlmA) |
| ACIJ: | JS278_01251(rlmAII) |
| TLA: | TLA_TLA_02693(rlmAII) |
| STRR: | EKD16_13820(rlmAII) |
| SACC: | EYD13_05275(rlmAII) |
| AMYZ: | HUW46_06367(rlmAII) |
| ACTU: | Actkin_00628(rlmAII) |
| MSAG: | GCM10017556_33270(rrmA) |
| SBAE: | DSM104329_00301(rlmAII) |
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| Reference |
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| Authors |
Liu M, Novotny GW, Douthwaite S |
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| Title |
Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Gustafsson C, Persson BC |
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| Title |
Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT |
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| Title |
Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Hansen LH, Kirpekar F, Douthwaite S |
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| Title |
Recognition of nucleotide G745 in 23 S ribosomal RNA by the rrmA methyltransferase. |
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| Journal |
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| Reference |
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| Authors |
Liu M, Douthwaite S |
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| Title |
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.1.1.187 |
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| LinkDB |
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