Purpose: Eucapnic voluntary hyperpnea (EVH) is a sensitive test for airway hyperresponsiveness (AHR). It is accepted by the IOC Medical Commission and used extensively in able bodied (AB) athletes to confirm the diagnosis of asthma. AHR has been shown in spinal cord injury (SCI), however EVH testing has not been reported. The purpose of this study was to compare airway responsiveness in two athletic populations; SCI rugby players and elite AB swimmers. Methods: Sixteen swimmers (AB) and twelve rugby players (SCI – tetraplegia) performed the standard EVH test. A drop of >10% in FEV₁ was considered a positive test. Results: Positive test incidence was 81% and 69% for the AB and SCI groups respectively. Although FEV₁/FVC was normal in the SCI group (84%), predicted FVC and FEV₁ were significantly lower (80%, 81%) when compared to AB (122%, 109%). Mean ventilations during hyperpnea were also significantly lower (SCI=95.9L/min, AB=154.5L/min). Conclusion: EVH testing in SCI produces prevalence levels similar to previous tests for airway hyperresponsiveness in this population. However, due to smaller ventilatory capacities, the EVH test may not be as sensitive a tool for the SCI population (compared to an AB population). Additional testing may be required.